STRidER STRs for identity ENFSI Reference database

The European Network of Forensic Science Institutes DNA Working Group (ENFSI DNA WG) provides an updated version to the previously published 'ENFSI DNA WG STR Population Database'. The concept of STRidER (STRs for identity ENFSI Reference database) is still based on the considerations detailed in 1, but the dataset was updated to comply with the technical developments and adaptation of additional STR markers 2.

The task of this website is to make STRidER generally available to forensic laboratories. This website can be used to calculate match probabilities of DNA profiles from high-quality cosmopolitan Caucasian populations across Europe. Additional populations will be added in the future. The online platform is work in progress and additional features will continuously become available 3.

This project was supported by HOME/2010/ISEC/MO/4000001759 (STEOFRAE).

STR loci and allele nomenclature

STRidER holds STR loci defined in the specifications of the ENFSI DNA WG. These include the STR loci D3S1358, VWA, D16S539, D8S1179, D21S11, D18S51, D2S441, D19S433, TH01, FGA, D22S1045, SE33, D10S1248, D1S1656, D12S391, and D2S1338. Additional loci may be included in the future based on the availability of high quality population data. Loci that are included in commercial kits but not in this version of STRidER, as no high quality population data are available, are dimmed in the input display.

As defined by the ENFSI DNA WG the following criteria for allele nomenclature are applied:

We defined the following allele category ranges taking the different allelic ladders into consideration:

VWA TH01 D21S11 FGA D8S1179 D3S1358 D18S51 TPOX CSF1PO D13S317 D7S820 D5S818 D16S539
10 - 23 3 - 14.3 23 - 38 16 - 47.2 7 - 19 11 - 20 7 - 28 TBA TBA TBA TBA TBA 7 - 16
D2S1338 D19S433 Penta D Penta E D1S1656 D2S441 D10S1248 D22S1045 D12S391 SE33 D6S1043 DYS391 Y indel
14 - 29 8 - 18.2 TBA TBA 9 - 20.3 8 - 17 9 - 19 9 - 20 14 - 27 5.3 - 38 TBA TBA TBA


1 Gill P, Foreman L, Buckleton JS, Triggs CM, Allen H (2003) A comparison of adjustment methods to test the robustness of an STR DNA database comprised of 24 European populations; Forensic Science International 131:184-96
2 Welch LA, Gill P, Phillips C, Ansell R, Morling N, Parson W, Palo JU, Bastisch I (2012) European Network of Forensic Science Institutes (ENFSI): Evaluation of new commercial STR multiplexes that include the European Standard Set (ESS) of markers; Forensic Sci Int Gen 6:819–826
3 Bodner M, Bastisch I, Butler JM, Fimmers R, Gill P, Gusmão L, Morling N, Phillips C, Prinz M, Schneider PM, Parson W (2016) Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on quality control of autosomal Short Tandem Repeat allele frequency databasing (STRidER); Forensic Sci Int Gen 24:97-102
4 Bär W., Brinkmann B., Budowle B., Carracedo A., Gill P., Lincoln P., Mayr W., Olaisen B. (1997), 'DNA recommendations. Further report of the DNA Commission of the ISFG regarding the use of short tandem repeat systems.', Forensic Science International 87(3), 179-184.